Nestin anticorps (AA 1464-1614)

N° du produit ABIN351499

Cet anticorps Souris Monoclonal détecte spécifiquement Nestin dans WB, IHC, ICC, IF, IHC (p) et IHC (fro). Il présente une réactivité envers Humain.

Aperçu rapide pour Nestin anticorps (AA 1464-1614) (ABIN351499)

Antigène: Nestin (NES)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Nestin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-Nestin anticorps

Épitope: AA 1464-1614

Marque: IHC-plus™

Specificité: This antibody is specific for human nestin. Species cross-reactivity: This antibody recognizes human nestin protein. It does not cross-react with rodent protein.

Aucune reactivité croisée: Rongeurs

Purification: Protein G purified

Immunogène: A fragment of the human nestin protein corresponding to residues 1464-1614.

Type of Immunogen: Protein

Isotype: IgG1 kappa

Information d'application

Indications d'application: Approved: ICC, IF, IHC, IHC-Fr, IHC-P (5 μg/mL), WB (1:1000 - 1:5000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: 1:1000-1:5000. Doublet band seen ~220-240kD.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C
Long term: -20°C.

Détails sur Nestin

Antigène: Nestin (NES)

Autre désignation: Nestin

Sujet: Name/Gene ID: NES

Synonyms: NES, Nestin, Nbla00170

ID gène: 10763

UniProt: P48681